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The Role of MreB in Intrabacterial Nanotransportation System for Cholera Toxin in Vibrio cholerae
https://ompu.repo.nii.ac.jp/records/320
https://ompu.repo.nii.ac.jp/records/320de52bf68-75b1-4f8d-b5fc-988d36930230
| 名前 / ファイル | ライセンス | アクション |
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bullompu68_1-2_3 TAKAYAMA.pdf (3.8 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2023-03-03 | |||||
| タイトル | ||||||
| タイトル | The Role of MreB in Intrabacterial Nanotransportation System for Cholera Toxin in Vibrio cholerae | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
TAKAYAMA, Shouichi
× TAKAYAMA, Shouichi× WU, Hong× SAKAGUCHI, Shoichi× FUJIOKA, Yoshihiko× SUZUKI, Youichi× NAKANO, Takashi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Vibrio cholerae possesses an intrabacterial nanotransportation system (ibNoTS) for transporting cholera toxin (CT) from the inner portion toward the peripheral portion of the cytoplasm. This system is controlled by extrabacterial pH and is closely associated with adenosine triphosphate. The transportation routes of ibNoTS for CT have not yet been examined in detail. To examine the routes of ibNoTS for CT, we demonstrated by immunoelectron microscopy that CT transported by ibNoTS localizes closely with the MreB filament in the bacterium, and the MreB filament polymerization inhibitor A22 obstructs ibNoTS for CT. The inhibition by A22 was also associated with CT secretion followed by ibNoTS inhibition, as determined by enzyme immunoassay. In support of this, we verified by enzyme immunoassay that CT interacted with the MreB filament. These findings indicate that CT along the route of ibNoTS is closely associated with the MreB filament. Since these phenomena were not observed in the other filaments, such as the FtsZ filament, we propose that the route of ibNoTS for CT is associated with the MreB filament in V. cholerae. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Bulletin of Osaka Medical and Pharmaceutical University 巻 68, 号 1-2, p. 17-28, 発行日 2022-12 |
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| 出版者 | ||||||
| 出版者 | Osaka Medical and Pharmaceutical University | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2436-4673 | |||||
