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Decreased Ionizong Radiation-induced DNA Damage Repair Function of Cultured Fibroblasts Derived from Patients with Xeroderma Pigmentosum Variant
https://ompu.repo.nii.ac.jp/records/280
https://ompu.repo.nii.ac.jp/records/280b429148d-e026-48b9-b041-3ca67a9d0f9f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
bullompu67_1-2_4 OTSUKA.pdf (2.2 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-02-08 | |||||
| タイトル | ||||||
| タイトル | Decreased Ionizong Radiation-induced DNA Damage Repair Function of Cultured Fibroblasts Derived from Patients with Xeroderma Pigmentosum Variant | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
OTSUKA, Toshihiro
× OTSUKA, Toshihiro× NAKAMURA, J. Asako× MORIWAKI, Shinichi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although it is obvious that patients with xeroderma pigmentosum (XP) have a deficient ability to repair ultraviolet radiation-induced DNA damage, the ionizing radiation (IR)-induced DNA damage repair function has not been elucidated. We sought to evaluate the post-IR DNA damage repair function in XP variant (XP-V) with deficient DNA polymerase η, which is possibly effect to the efficient DNA damage repair responses. We assessed the post-IR DNA repair ability in skin fibroblasts derived from 11 XP-V patients using immunofluorescence staining with an antibody to phosphorylated H2AX, which is a marker of DNA double-strand break. The focus formation of XP-V cells after radiation was compared to that of normal and ataxia telangiectasia cells. The postIR DNA damage, in any group, was most prominently determined in the 30 minutes after radiation exposure and recovered over the next four hours. Post-IR DNA damage repair occurred immediately after radiation exposure in XP-V cells as it did in normal cells; however, the rate of DNA repair was decreased in all XP-V cells. These results suggest that the DNA damage repair function of XP-V patients was impaired after IR exposure. To further analyze their radiation-induced DNA damage repair function is important. |
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| 言語 | en | |||||
| bibliographic_information |
en : Bulletin of Osaka Medical and Pharmaceutical University 巻 67, 号 1-2, p. 25-29, 発行日 2021-12 |
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| 出版者 | ||||||
| 出版者 | Osaka Medical and Pharmaceutical University | |||||
| 言語 | en | |||||
| item_10002_source_id_9 | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2436-4673 | |||||
